At least four polyphosphate moieties are required for negative staining by DAPI. Likely, the negative staining associated with IP7 is due to the highly negative charge of the fully phosphorylated inositol ring that, with the addition of the pyrophosphate moiety, results in DAPI photobleaching. We have found that DAPI and Toluidine Blue stains can be sequentially performed on the same gel; however the Toluidine Blue staining looses sensitivity. In conclusion, the current study demonstrates that both FR and MPred treatments result in loss of body mass and muscle mass during a 14 d intervention. However, muscle mitochondrial function was largely unchanged in oxidative and mixed oxidativeglycolytic muscles following both FR and MPred. The maintenance of mitochondrial function occurred at the same time there was a ,40–50% decline in the rate of synthesis of mitochondrial proteins in the same muscles. It is not yet known whether mitochondrial proteins are differentially targeted for breakdown under these conditions or if other compensatory mechanisms may explain the maintenance of mitochondrial function. The finding that the decline in muscle protein synthesis was similar in FR and MPred rats highlights the importance of accounting for changes in food intake in rats receiving glucocorticoids. The CV of SS firing of PCs recorded in vivo is reported to be quite high : close to or even higher than 1, the CV of a Poisson process. Conversely, PCs in the in vitro slice preparation fire very regularly. To test whether this difference in firing properties is as large as is commonly assumed and to investigate its possible functional importance, we analyzed the fine-temporal structure of SS trains in different preparations and behavioral states in more detail, focusing on the short-term variability.We assume that the number of individuals traveling from the source region to the at-risk country each day is known. Here, we investigated the mechanism underlying the increased sensitivity to oxidative stress of frataxin-depleted cells. The Nrf2-dependent signaling pathway was found to be defective. The phenotype associated with the Nrf2-signaling defect was corrected by the catalase mimetic Euk134, emphasizing the key role for the cellular hydrogen peroxide content.The probability that a randomly selected traveler is a recently-infected person is taken to be equal to the prevalence of recently-infected people in the source region on that day. The incidence of infection in the source region is assumed to grow exponentially initially, with the rate of exponential growth determined by the disease reproduction number and the serial interval. We note that about ten-fold more enzyme is necessary to detect the tellurite reductase activity of these catalases than to detect their MDV3100 dismutase activities in situ. This result is consistent with the observation that the NADH oxidase reaction mediated by bacterial catalases has a much slower turnover rate than does the dismutase reaction.