Similar to other antifungals, there have been increased incidences of resistance to azole antifungals in A. fumigatus. The echinocandins have been most promising but are available only as parenteral formulations. Despite the continuous advancements in the field of antifungal drug discovery, the mortality rate due to IA remains high. Therefore, the development of an ideal antifungal drug remains challenging and elusive. This has driven the work on discovering novel and effective antifungal molecules, from natural and synthetic sources, which will target pathway different than those targeted by current drugs to exert specific activity with minimal or no side effects. The molecules belonging to class of coumarins have Mogroside-IV attracted considerable interest in recent years, owing to their diverse pharmacological properties. In spite of increasing mortality, therapeutic options for the treatment of aspergillosis have been limited to few classes of antifungals. Therefore, it is imperative to develop and characterize new molecules with no or minimal side effects for the effective management of disease. In an effort to explore potent anti-Aspergillus molecules, present study was aimed to examine the safety and antifungal efficacy of SCD-1, a synthetic coumarin derivative using mouse model. Although coumarins have been explored for their Isoastragaloside-I biological properties such as anti-inflammatory, cytotoxic, anti-tubercular and angiogenic etc, studies focusing on their antifungal efficacy are limited in number, only in vitro antifungal activity has been indicated in some reports. Till date, there have been no reports on the toxicity and in vivo antifungal activity of coumarins. We have previously reported the in vitro antifungal activity of SCD-1, and its impact on the proteomic machinery of A. fumigatus. The present study addressed for the first time the in vivo safety and antifungal efficacy of a synthetic coumarin derivative. The analysis of safety of a compound is a crucial step in determining its biological property. The adoption of OECD guidelines for acute toxicity testing has been an important landmark in animal welfare.