This corresponds with another study showing that in some soybean varieties both PRP1 and PRP2 proteins are smaller because of in frame deletions in the coding region of units of the tandem decamer repeats. In previous reports, PRP1 and PRP2 had also been reported to be linked, but separated by approximately 13% recombination units. A BLAST search for these genes in Phytozome showed that they are located on chromosome 9, approximately 146 kb apart. As described in our results, the expression of PRP1 was dramatically higher in the yellow Harosoy isolines, as compared to black Clark isolines. Likewise, another study showed that the I locus which controls inhibition of anthocyanin accumulation in the epidermal cells of the soybean seed coat also affects abundance of PRP1 mRNA and protein in the seed coat. Interestingly, an epistatic interaction between the recessive i and t alleles also causes cracking of the pigmented seed coat. This seed coat cracking is not related to the defective seed coats of the net pattern described in this report which is Bosentan independent of seed color as shown in Figure 1. However, this genetic interaction implies an interaction of the flavonoid pathway with cell wall CK-636 structure as the t locus is known to encode a flavonoid 39 hydroxylase. Our RNA-Seq data show excellent agreement with the previous RNA blots indicating a delay in the decline of PRP1 transcripts in the defective seed coats leading to higher levels in the defective seed coats at the middle weight range of 100�C 200 mg. Despite the presence of significant levels of PRP1 transcripts in the defective seed coats, no PRP1 protein was detectable by immunoblotting in defective Clark seed coats at any stage of seed development but it was easily extractable from the standard, non-defective isoline at the same stages. However, the similar PRP2 protein was extractable from both standard and defective seed coats. These results implied that a major physiological event in the net pattern defective seed coats may be the irreversible cross-linking of PRP1 into the cell wall occurring in the developing seeds. Transcription factors are important players for controlling the flow of genetic information from DNA to RNA and ultimately affecting the growth and physiology of the plant. In this study, there were approximately 240 differentially expressed transcription factor genes at different seed weight stages.