This B. villosa RNAseq pattern was distinct and somewhat unexpected since the BvEGL3 expression was lower than in glabrous B. oleracea leaves and the BvTTG1 expression was quite similar in level to BoTTG1 expression. Expression of the BvTRY-1 and BvCPC-1 was also high in hairy B. villosa, and expression of BoTRY-1 was high in glabrous B. oleracea leaves. This was inconsistent with the Arabidopsis model of TRY and CPC as Doxycycline hydrochloride negative regulators of trichome initiation, where enhanced leaf trichome density phenotype occurred when TRY expression was knocked down in Arabidopsis try mutants. The data implies that BvTRY-1, BvCPC-1, and BvETC3-1 genes may not behave as negative regulators of trichome initiation in B. villosa. Protein coding sequences for BvTRY-1 and BvTRY-2 genes were closest to those of non-hairy BoTRY-3 and BnTRY, and all four of these are closer to each other and to A. thaliana than to the other Brassica TRY genes. Redundant trichome negative regulatory genes exist in the A. thaliana model and functional redundancy can speed up gene evolution. Hence, B villosa may use these R3 regulatory genes with high expression for a different purpose in the densely covered B. villosa true leaves. This hypothesis is supported by the insertion of BvTRY-1 into B. napus, yielding transgenic plants in which trichome density is not affected even though the same binary construct was used to depress Arabidopsis trichome development. In the future, it will be particularly useful to express these B villosa genes in a range of other Brassica species and to develop knock-out RNAi lines in B. villosa to solve the mystery of their true function. This will depend on the development of a transformation system for B. villosa, such as the protocols that now exist for B. napus, B. rapa, B. oleracea, and B. carinata. Additional analysis of B. villosa gene structure is also necessary for a complete understanding of their introns, untranslated regions, and promoters. For example, intron 1 and 30 non-coding nucleotides in A. thaliana are important for the expression of the GL1 gene. A 620 bp fragment of the TRY D4476 promoter contains sequences that mediate the repression of its own expression, and deletion of this promoter region can rescue the A. thaliana try mutant phenotype.