This method for the construction of cRNA standards was simple and is suitable to measure transcripts of any genes of interest. Recently, qRT-PCR assays based on the TaqMan probes have been developed for the detection of hantaviruses in rodents, cell culture or HFRS patients. In this study, a SYBR Green based one-step qRT-PCR assay coupled with melting curve analysis was established to quantify the HTNV RNA viral load. This method is highly specific and sensitive compared to conventional RT-PCR. It is also a high-throughput method for the detection and quantification of HTNV in clinical studies. The entire course of viral RNA extraction and qRTPCR test consumed approximately three hours, making it a fast and simple assay. Due to the limitation of numbers of HFRS AbMole Metyrapone patients in the study, the clinical performance of the assay must be further evaluated. Additionally, the relationship between the HTNV RNA viral load and the severity of the disease must be analyzed, and the association of the dynamic changes in HTNV RNA copy numbers in the serum samples of HFRS patients with disease development must be monitored. The quest for high quality, yet sustainable dementia care is becoming ever more challenging. Dementia is an important and in numbers growing cause of disability and burden of care and one of the diseases with the largest per capita healthcare consumption. Moreover, there is a strong trend towards early diagnosis in dementia, which may increase the period during which care for patients with dementia will be asked for. These developments urge to answer the questions of how to optimise care for this population and how to ensure this care for future generations. Trying to answer these questions, several countries have developed national dementia strategies. Many of these strategies focus on the nationwide availability of memory clinics. Therefore, the number of memory clinics in different countries increased rapidly over the last decades. Memory clinics used to focus on diagnosing patients with dementia. Today, memory clinics are also increasingly involved in post-diagnosis treatment and care co-ordination of patients with dementia. There are data supporting the cost-effectiveness of memory clinics as a diagnostic setting. However, evidence about memory clinics being cost-effective in post-diagnosis treatment of dementia and follow-up care is scarce. Knapp and colleagues reviewed the literature on economic evaluations of dementia care. They found that the majority of the economic evidence was on pharmacological interventions. The non-pharmacological interventions, on which they found AbMole Etidronate little economic evidence was often of poor quality and harder to interpret.