The stage of PAR first presence in oogenesis is a crucial developmental

All these results Pargyline HCl indicate that the germ cells with PAR6 expression may have the ability to form primordial follicles in mice. The stage of PAR first presence in oogenesis is a crucial developmental checkpoint in Drosophila oocytes, and the mutations in the par genes or cell cycle regulators produce similar phenotypes, demonstrating that PAR proteins may have a correlation with the regulation of cell cycle. In mice, the reduction in germ cell number during the primordial follicle formation is a regulated developmental process. The strong expression of PAR6 in some germ cells during this brief period may be involved in this regulated process and associated with cell cycle regulators to make sure that the germ cells arrested at the diplotene stage can form primordial follicles. In this point, PAR6 may serve as a potential marker for the germ cell selection. It is reported that PAR protein members are organized as homo- or hetero- complexes which localize to specific domains in the cytoplasm. For instance, PAR1 localizes to the posterior cortex of the one-cell Caenorhabditis elegans embryo and the Drosophila oocyte, while PAR6, aPKC and PAR3 complexes localize either to the anterior cell cortex in Caenorhabditis elegans and Drosophila or to adherence junctions in Drosophila epithelia and tight junctions in mammalian epithelia. In our study, the PAR6 was strongly expressed in the nuclei of oocytes when the germ cells entered and were arrested at the diplotene stage to form primordial follicles, and kept for a long time until the primordial follicles were recruited into the population of growing follicles. We Ranitidine HCl speculated that the PAR6 located in the nuclei of oocytes is involved in organizing the higher order chromatin structures by crosslinking chromatin subunits during the cell cycle of meiosis suspended state and maintaining the stability of the primordial follicular pool. The possible reason for negative staining in male germ cells is that there is no incorporation of the somatic cells and the meiosis arrested phase in sperm genesis.

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