The T cell populations showing these unconventional cytokine profiles accumulated uncharacterized proteins

Our data suggest that 170,377 SNPs is unique to G3116 and 37,380 SNPs is unique to DV92 ; this provides an opportunity to study the wild winter and cultivated spring habits of the two accessions in greater detail. The SNP and SSR genetic sites identified in our dataset, along with those identified in other genetic populations and wheat projects, will provide useful marker resources for fine mapping experiments and marker-assisted wheat breeding programs. Along with the T. monococcum transcriptomes from two accessions, we have provided additional genomic and genetic resources including their functional annotations, differential gene expression analyses and potential SNPs and SSRs, which can be used to explore Triticeae genome diversity, co-expression networks involved in photomorphogenesis and to develop stochastic and metabolic networks. In addition, these resources can be used to identify novel genes, transcript models and eQTLs, and to study plant’s adaptation to diverse climatic conditions, impacts of domestication on crop plants and evolution of novel genes. Th1 and Th2 cells play important roles in the immune response to many infectious diseases and in autoimmune disorders. Th1 and Th2 cells mutually impede their generation, and Th1and Th2-related cytokines are not thought to be simultaneously secreted from single helper T cells. However, it was recently reported that IFN-c-producing Th1 cells inherently possess the capacity to convert their cytokine productivity. Th1 cells stimulated by IL-18 and antigen acquire the potential to produce several Th2-related cytokines, including IL-13, but not IL-4, as well as IFN-c. Th1 cells which gain productivity of Th2 cytokines are termed “super Th1 cells”. Indeed, within the IL-18induced super Th1 cells, Gata3 and T-bet, which are the crucial transcription factors for the induction of Th2 and Th1 cells, respectively, coexist. Whilst some recent studies demonstrate that one transcription factor, promyelocytic leukemia zinc finger, which was originally identified as a partner fused with retinoic acid receptors in acute promyelocytic leukemia, is indispensable for the dual secretion of IFN-c and IL-4 from cd T cells or NKT cells. It has been also reported that exogenous PLZF leads to the concomitant production of IFN-c and IL-4 from single T cells upon TCR stimulation. Since PLZF-transgenic T cells seem to convert their nature from differentiated mature types into ‘innate’ types, PLZF might be involved in the plasticity of committed T cells, such as Th1 and Th2 cells. Very recently, we reported that some conventional CD4 + T cells acquire atypical cytokine production capacities, producing combinations of “IFN-c+IL-13” and “IFN-c+IL-4”, during Schistosoma mansoni infection. Furthermore, some of these unique populations displayed the potential for secreting three cytokines concomitantly.

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