The PMI- 1 mimic peptide could be used as a predictive biomarker to guide optimal postoperative management for patients undergoing CABG. In addition, it may serve as a new basis for exploration into the molecular mechanisms underlying PMI pathogenesis. The identification of PE susceptible variants can provide new insight into its etiology. Moreover, it is an important step to individualize treatment and prevention programs according to the genetic profiles and/or clinical manifestation. The search for susceptible genes has led to an increased number of published studies associating genetic factors with PE. However, attempts to replicate these findings yielded inconsistent results. In a metaanalysis including 192 genetic association studies,RA-2 replicated genetic variants were identified. Another meta-analysis identified 542 genetic association studies and included 22 independent meta-analyses. But both studies did not include TGF-b 1 gene in the meta-analysis. Our results showed that the missed 869 T.C gene was significantly associated with risk of PE. The reported mean plasma TGF-b 1 level ranged from 18 pg/ mL to 20.3 ng/mL in normal pregnancy women. This considerable variability may be due to the differences in procedures for obtaining and processing blood samples, in the TGF-b 1 analysis kits, as well as in population characteristics. Platelet is a rich source of TGF-b 1,LUF5834 and platelet degranulation could happen during plasma preparation, leading to overestimation of the TGF plasma level. Two included studies used platelet-depleted plasma and others did not indicate whether platelet was depleted. Leukocytes also contain a large amount of TGF-b 1 and it can be speculated that during plasma preparation, leukocytes secrete TGF-b 1 into the plasma. Therefore, different procedures for plasma preparation between studies are a potential source of variation in reported TGF-b 1 levels. The assay methodology could be another important source of variation. Difficulties of TGF-b 1 measurement in complex biological fluids were discussed in detail in the review of Grainger.