However, for the analysis of the blood leukocyte we used a small dataset of gene expression that is publicly available for comparison to the cortical predictions and between MCI and AD patients. A further analysis in the future utilizing richer gene expression datasets from blood cells of AD and MCI patients will aid to Procyanidin B4 support this study��s findings. Both analyses shared several pathways whose activity significantly increased in the blood and Almotriptan Malate decreased in the brain, implying a possible compensation mechanism. Moreover, we predict biomarkers that are common to both analyses, strengthening the potential of these metabolites as candidates for early diagnosis of AD. The MTA analysis yielded predictions of drug targets that may reverse the metabolic state of the disease back to the healthy one. Vitamin D and steroid metabolism appear in our analysis to be important in reversing the metabolic state in the disease. Furthermore, although it did not pass the FDR cutoff, our findings may hint to the importance of cholesterol in the pathogenesis of AD and the potential value of keeping its levels in check. The use of MTA for finding potential drug targets holds an advantage for finding drug candidates that act globally to reverse the entire metabolic network state to the healthy state, and thus may have lesser side effects. Our analysis is in line with the common view that metabolism is overall decreased in AD. Several transport pathways appear throughout our analyses, further emphasizing the importance of metabolite transport in the disease. The predicted candidate biomarkers and drug targets that were discovered in this analysis may offer new metabolic leads for advancing the diagnosis of AD and its treatment. Hopefully, this work will motivate and guide future experimental studies geared at studying some of these leads. A fundamental goal of stem cell biology is to understand the mechanism by which stem cells select particular pathways of differentiation. Embryonic stem cells provide a surrogate for in vivo development, enabling the analysis of multilineage differentiation within an in vitro environment.