In some cases, this accumulation can lead to the transcriptional silencing of the overlapping genes. This system seems to preferentially target inducible genes, as for instance the GAL system. The JEN1 genomic region has been shown to be able to produce two stable unannotated transcripts in sense and antisense positions. Moreover, it overlaps with one large Xrn1 sensitive lncRNA antisense to the JEN1 mRNA sequence. Therefore, it was tempting to speculate that the negative effects of Dhh1 and Pat1 deletion on JEN1 expression in acetic acid could be mediated by an accumulation of one or several of these intergenic or antisense lncRNAs. Northern blot analyses of the three non coding RNAs overlapping the JEN1 locus could not show any difference of expression between the wild type and the dhh1 mutant grown in acetic acid. This suggested that Dhh1 does not act on JEN1 expression in acetic acid by degrading overlapping transcripts. This is consistent with previous observations that Dhh1 and Pat1 had no role in the transcriptional silencing by the accumulation of lncRNAs. More generally, we pointed out about 900 potential targets for Dhh1, which are involved in many, different cellular pathways. These results emphasized the large role of Dhh1 in gene expression regulation. Still, this number is Tiliroside relatively small, considering that Dhh1 participates to a general mRNA degradation pathway. Interestingly, in trypanosomes, microarray analyses of dhh1 mutants suggested that it Garcinone-D controls the expression of only 1% of the genes, several of them being involved specifically in developmental processes. More recently, CLIP-seq experiments have shown that Dhh1 was able to bind about 300 mRNAs in standard growth conditions. Our microarray results and the model of JEN1 regulation discussed above support the idea that, besides its general role in the global cytoplasmic mRNA decay, Dhh1, like Xrn1 or Dcp2 may have more specific roles in the post-transcriptional and/or transcriptional regulation of some genes, in response to environmental stimuli.