Where conserved sites with discriminating SNPs are few and far between, can be successfully tackled. However, since our method does not correct for the decline of signal intensity in long pyrosequencing reads, it may not perform well on assays in which long amplicons need to be sequenced. We postulate that the method presented in this paper will lead to a more wide-spread adaptation of multiplex pyrosequencing in diagnostics, as it allows the quick and cost-effective generation of high-quality results. Adipocytes not only store excess energy, but also function as endocrine cells that take part in the regulation of energy homeostasis. Obesity, characterized by excess energy stored in white adipose tissue, reflects the cumulative sum of the excess energy intake over energy expenditure over time. The sympathetic nervous system innervates both WAT and interscapular brown adipose tissue, and is the primary initiator of lipolysis.However, 40 women in this study experienced late recurrence after 5 years despite endocrine therapy. Late recurrence despite endocrine therapy has been a challenging obstacle to overcome. Retrospective design is a major limitation in this study. This study could be affected by the bias associated with the decision of endocrine therapy or the inherent selection bias of retrospective studies. In addition, human epidermal receptor-2 status was not evaluated in this study because the routine IHC test for HER-2 was not done in the investigated period. Moreover, the grade of ER positivity could not be assessed in this study. The ER grade is reported to be related with endocrine responsiveness and associated with survival outcome of ER-positive patients. Because of the absence of information on HER-2 status and ER grade, further studies to define biologic effect of these factors on late recurrence still remains an unmet need. Future work with the identification of novel biomarkers as well as HER-2 and ER grade could support and strengthen our findings regarding early and late recurrence in ER-positive women. In the article, the dissimilarity in prognostic factors between early recurrence and late recurrence in ER-positive disease was noted. Above all, this investigation highlights the diluted effect of nodal stage on late recurrence compared with early recurrence, and it suggests that tumor biology plays a more important role than tumor load in late recurrence of ER-positive disease. Our results propose that comprehensive consideration of both tumor load and tumor biology is needed to perform the translational research involving parallel comparison between early and late recurrence in this subset of patients. The ovary is an important organ for oocyte formation and release. In rodent species, the ovary is encapsulated by a thin membrane structure that fused with the end of the oviduct, which is called the ovarian bursa. The ovarian bursa shields the ovary from the peritoneal environment and provides a fluid chamber for oocytes development and ovarian function. Upon ovulation, the oocytes are expulsed into the ovarian bursa along with ovarian fluids.